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TABLE OF CONTENTS
Summary
Introduction
Methods
Results
 THP cell lines
 3T3 cell lines
 B16 cell lines
 Rafi cell lines
Discussion and frequency model
 Discussion, Part 1
  THP cell lines
  3T3 cell lines
  B16 cell lines
  Rafi cell lines
  Reproducibility
  The cooperativity effect
  of the frequencies
  Prototype and series instrument
  of F-SCAN 2
  Regarding the signal characteristics
  of the individual frequencies
 Discussion, Part 2
  Are the described EMF effects
  due to mutations?
  Model for the cellular effects
  by EMF treatment
Appendix
 Literature
 Abbreviations
 Author
  

Low-energy electromagnetic frequencies induce
conspicuous, reproducible, and lasting effects in human
and animal cells

ANHANG

Literature
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Abbreviations
BSA bovine serum albumin
EM electromagnetic
EMF electromagnetic frequencies
Frequenzen definition of EMF A, B, and C: see methods
Hz Hertz, frequency per second
FBS fetal bovine serum
alpha-MSH  melanin-stimulating hormone
NBS newborn bovine serum
PMA phorbol ester
SE seeding efficiency
3T3-L1 untreated mouse fibroblast cells, pre-adipocytes
3T3-J 3T3-L1 cells treated with EMF group A+C
3T3-K 3T3-L1 cells treated with EMF group B
B16-F1 untreated mouse melanoma cells
B16-J  B16-F1 cells treated with EMF group A
Rafi untreated primary rat fibroblasts
Rafi-J Rafi cells treated with EMF group A
Rafi-K Rafi cells treated with EMF group B
THP-1 untreated human monocytes
THP-J THP-1 cells treated with EMF group A+C

 

Author

Dr. Jean Samuel Burckhardt
Unterer Zielweg 24
CH-4143 Dornach
Switzerland

Dr. Jean Burckhardt performed these experiments alone from 2005 to 2009 at the Department for Biomedicine, formerly the Zentrum für Lehre und Forschung (ZLF), Hebelstrasse 20, Kantonsspital Basel, 4031 Basel (Switzerland), in the research department of Professor Dr. Alex N. Eberle. This work was only possible thanks to the extraordinarily generous support by Alex Eberle and his open-mindedness regarding this project. We would therefore like to thank Alex Eberle and all other colleagues who assisted us in many aspects of this work, in particular Martine Calame (cell culture, melanin assay) and Verena Jaeggin (flow cytometry).